| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 2x1h,1x30min |
| Standardrange | cut-offindex |
| Specimen/Volumes | 10µLserum |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
Toxoplasmagondiiisasmallintracellularparasite,whoselivecyclehasasexualandanasexualphase.Sexualdevelopmentisrestrictedtotheintestinalcellsof(probablyexclusively)cats;theoocystsformedareexcretedandduetotheirresistantcellwallstheymaybeinfectiousunderadvantageouscircumstancesforatleast1year.AnimalsandmanareintermediatehostsfortheasexualproliferationofT.gondii:theingestedparasiteswillproliferateexplosivelywithinthehostcellslysingthemeventually.Theydisseminatethroughoutthebodyviacirculationandlymphaticsystemandthoughmayinfectanycelltype.Inmuscleandbraincellscystsareformedwhicharespheroidalandabout5-100µmindiameter.Cystsarevirtuallyimmortalintheintermediatehost.Toxoplasmagondiiisthemostcommonparasiteinhumans,butitsabundance(7-80%)ishighlydependentonthegeographicarea,thesocioeconomicstatusandthenutritionalcustoms.Infectiononlyrarelycausestoxoplasmosisandusuallyclinicalsymptomsareabsent,butmayproducesevereproblemsinimmunosuppressedpersonsandfetus.Becauseonlyaprimaryinfectionduringpregnancymaybedangerousandevenfatalfortheunborn(theprobABIlityofcongenitalinfectionisabout50%),therecentonsetofaninfectionmustbeexcluded.Inpregnantwomeninover98%ofcases,theabsenceofIgMexcludesthepossibilityofrecentinfection.Innewbornstheverypresenceofanti-toxoplasmaIgMissufficienttoconfirmacongenitaltoxoplasmosis,sincematernalIgM,unlikeIgG,doesnotcrosstheplacentalbarrier.ButasignificantnumberofinfectedinfantsdonotdevelopdetectableIgMlevelsandthusarefalsenegative.Inimmunosuppressedpatientstoxoplasmosiscausesseverecomplicationsmostlybyreactivationofanearlierlatentinfection.Infectionmaybeidentifiedby
- PCR
- Indirectimmunofluorescence(IIF)
- SEROlogy:DetectionofantibodyproductionbyELISA
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