| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h,1x30min,1x15min |
| Standardrange | cut-offindex |
| Specimen/Volumes | 10µLserum,plasma |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
EnzymeimmunoassayforthequalitativedeterminationofIgMclassantibodiesagainstMumpsvirusinhumanserumorplasma(citrate).MumpsvirusesareRNAvirusesofthefamilyParamyxoviridae.Thevirionsaresphericalparticlesof150-250nmindiameterconsistingofaribonucleoproteinwithhelicalsymmetryandenvelopedbymatrixproteinandalipidbilayerwhichcontainstwospikelinestructures:viralhemagglutinin(H)andviralneuraminidase(N).Mumpsvirusinvolvesprimarilytheparotidandrelatedsalivaryglands;howeverinfectioncanleadtoCNSdiseaseandaccumulationofthevirusinCSF.Mumps(EpidemicParotitis)isanacutecontagiousviraldiseasemostlyoccuringinchildren.Nearly50%ofallinfectionsaresubclinical.Thehighestincidenceofclinicalmanifestationsisfoundintheagegroupof4to15years.Secondaryinfectionsarerarebecauseoflong-lastingimmunity.10to35%ofmumpscasesdeveloporchitiswhichoccursnearlyalwaysafterpuberty.Theprocessismostlyunilateralandtheprognosisusuallygood.MumpsvirushasbeenoneofthemostimportantcausesofviralCNSdisease(meningitisandencephalitis)inUSA;vaccineadmiNISTrationhasgreatlyreduceditsincidence.
Thepresenceofvirusresp.infectionmaybeidentifiedby:
- Complementfixation,Hemadsorption,Hemagglutination-Inhibition
- DetectionofantibodyproductionbyELISAMeasurementofIgG/IgMantibodiesintheserumallowsforthedeterminationoftheimmunestatusofthepatientsandrecommendationsaboutthenecessityofimmunization.
