Phospholipid-8-Profile-AbIgAELISAisasolidphaseenzymeimmunoassayfortheseparatequalitativedetectionofIgAantibodiesagainstphospholipidsinhumanserum.Theassayemployshighlypurifiedhumanß2-GlycoproteinI,Cardiolipin+ß2-GlycoproteinI,CardiolipinandPhosphatidylcholin,-ethanolamin,-inositol,-serineandSphingomyelin.Theassayisanaidinthediagnosisandriskestimationofthrombosisinpatientswithsystemiclupuserythematosus.Antibodiesagainstphospholipids,componentsoftheBIOLOGicalmembranes,arespecificforphospholipidssuchasCardiolipin,Phosphatidyl-inositol,-ethanolamine,-choline,Sphingomyelinandphosphatidicacid.Anti-phospholipidantibodiesarefrequentlyfoundinseraofpatientswithsystemiclupuserythematosus(SLE)andrelateddiseases.Theoccurrenceofanti-phospholipidantibodiesinpatientswithSLEandrelateddiseasesistypicalforasecondaryanti-phospholipidsyndrome(APS).Incontrast,antiphospholipidantibodiesinpatientswithnootherautoimmunediseasescharacterizetheprimaryAPS.Manystudieshaveshownacorrelationbetweentheseautoantibodiesandanenhancedincidenceofthrombosis,thrombocytopeniaandhABItualabortions(asaconsequenceofplacentalinfarct).Theexactmechanismsbywhichpathogenicanti-phospholipidantibodiesinducethrombosisisnotyetrevealedfully.