Laminin-AbELISAisasolidphaseenzymeimmunoassayemployinghighlypurifiednativehumanlaminin-1fortheseparatequantitativeandqualitativedetectionofIgGantibodiesagainstlaminin-1inhumanserum.Antibodiesagainstlaminin-1canbefoundinpatientswithrecurrentabortionandinfertilityassociatedwithendometriosis.Laminins,multifunctionalglycoproteinsofthebasementmembrane,areinvolvedindiverseBIOLOGicalactivities,includingthepromotionofcelladhesion,migration,proliferationanddifferentiation,aswellastheformationofthescaffoldingnetworkinbasementmembranes.Todate,atleast15differentisoformsoflamininhavebeenidentifiedandareknowntodisplaytissue-specificexpressionduringdifferentstagesofdevelopment.Laminin-1,composedofa1,b1andg1chains,istheearliestsynthesizednetwork-formingcomponentduringembryogenesisandplaysanimportantroleinembryonicdevelopment,embryonicimplantationandplacentation.Inblastocytesorearlyimplantingmouseembryo,laminin-1islocalizedintheinnercellmassandtrophectodermbasementmembrane.Asimplantationproceeds,laminin-1isexpressedinchorionicbasementmembraneandinReithert`smembranenearectoplacentalcone.RecenthumanstudiesindicatethatIgGautoantibodiesagaiNISTlaminin-1aresignificantlyassociatedwithrecurrentfirst-trimestermiscarrages.Inanimalmodels,activeimmunizationwithmouselaminin-1causedabortionsinanti-laminin-1positivemice.Itwassuggestedthatanti-laminin-1antibodiesmayhaveaharmfuleffectoneventsatearlystagesofpregnancy,suchasembryonicimplantation,embryogenesis,placentalvascularization,and/orplacentalnutrienttransport.MoroverrecentclinicalstudiesalsoshowedthatIgGlaminin-1antibodiesaresignificantlyassociatedwithendometriosisininfertilepatients.Thus,measurementofIgGanti-Laminin-1autoantibodiesmaybeausefultoolfordiagnosingreproductivefailure,suchasrecurrentabortionandinfertilityassociatedwithendometriosis.