| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h,1x30min,1x15min |
| Standardrange | cut-offindex |
| Specimen/Volumes | 10µLserum |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
Entamoebahistolyticaisananaerobeparasiteformingcystswhichhavefoursmallnucleiandmeasure10-15μmindiameter.Thecystsaresturdyandresistadverseenvironmentalconditions.AfteringestionbyasusceptIBLehost(invertebratesandvertebratesincludinghumans),itswallisdisruptedbytheformationofasmallopeningthroughwhichanamoebaemerges.Theamoebadividesseriallythroughthreecyclesgivingrisetoeightuninucleatetrophozoitesfromonecystwhicharemotileandmeasure20-30μmindiameter.Someofthetrophozoitestheninvadethetissuesofthelargeintestineandmayerodethemsoextensivelythattheygainentranceintothebloodstream.Thus,amoebaecanreachallpartsofthebody.InfectionwithEntamoebahistolyticahasworldwidedistribution.ItisthecausativeagentofamoebiasisandamoebicdysenteryandinhABItsthelumenandmucosaofthelargeintestine,predominantlythetransversecolonandcecum.Extraintestinalamoebiasiscanafflictanyorganortissue.Themajorityofinfectedindividualsarefreeofsymptoms;thishighincidenceofasymptomaticcarrierscomplicatesmatters.Thosewhoaresymptomaticexperienceawiderangeofmanifestations.Membersofallagegroupsandbothsexesareinfected.Theriskofinfectionincreaseswithinadequatesanitaryconditions.Anincreasedprevalenceofamoebiasisisfoundamongpeople,whohaveanincreasedriskofexposureintheagriculturaloccupationsandinmalehomosexuals.Infectionmaybeidentifiedby
- Microscopy:stoolexamination:ironhaematoxylinmethod,merthiolatiodformolconcentration(MIFC)
- SEROlogy:CF,CIE,ELISAInfectionmaybeidentifiedby
- Microscopy
- Serology:IFA,ELISA
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