Kitsize | 12x8 |
Method | ELISA |
Incubationtime | 1x1h,1x30min,1x15min |
Standardrange | cut-offindex |
Specimen/Volumes | 10µLserum |
Substrate/isotope | TMB450nm |
RegulatoryStatus: | EU:CE |
Chlamydiaearenonmotile,Gramnegativeandobligatoryintracellulargrowingbacteriawhichformcharacteristicinclusionswithinthecytoplasmofparasitizedcells.TheyareeasilyvisIBLeinthelightmicroscope.ThreedifferentChlamydiaspeciespathogenicforhumansareknown:Chlamydiatrachomatis,ChlamydiapneumoniaeandChlamydiapsittaci,andonespeciesonlypathogenicforanimals(C.pecorum).Chlamydiatrachomatisisthemostprevalentagentofsexuallytransmitteddiseasesworldwide(400-500millioncases)andthenumberofinfectionsisconstantlygrowing,duringchildbirth,causingconjunctivitisorpneumoniainnewborns.Untreatedcasesofchlamydialinfectioncanleadtochronicsalpingitis,possiblyresultinginectopicpregnancyorinfertility.Inmales,C.trachomatisisamajorcauseofnon-gonococcalurethritis.AsevereprobleminChlamydiainfectionsisthefrequentasymptomaticinsidiouscoursewhichmayresultintheinitiationofchronicdiseases.Inmanyinstancesprimaryinfectionsarenotrecognizedandonlythesequelaecausedbyascended,persistingagentsarediagnosed.Infectionmaybeidentifiedby
- Microscopy:Giemsastain
- PCR
- SEROlogy:DetectionofantigensbyELISA
- DetectionofantibodiesbyIF,EIA,ELISA