| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h,1x30min,1x15min |
| Standardrange | cut-offindex |
| Specimen/Volumes | 10µLSerum,Plasma |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
About30millionpeoplein70countries,primarilyinwarmerclimates,areinfectedwiththeroundwormStrongyloidesstercoralis.Typicalsymptomsincludeserpiginousskinlesions,respiratoryproblemsandatypicalgastrointestinalcomplaints.Immunocompromisedpatientsareataparticularriskbecauseimmunosuppressionmayprecipitatealife-threateninghyperinfectionsyndromewithamortalityrateofover80%.
Testmethodsincludelarvalmigrationtestswithstoolsamplesorthedetectionofserumantibodies.Laboratorydiagnosisisdifficultbecauseasmanyas6stoolsamplesareoftenrequiredtodetectthelarvae.FurThermore,conventionalimmunoassaysdisplayahighdegreeofcross-reactivitywithotherhelminths.Testsystemswithhighspecificityarethereforeofgreatimportanceinthediagnosisandtherapymonitoring.
Byusingrecombinantantigens,ourELISAforthedeterminationoftotalantibodiesagainstStrongyloidesstercoralisallowsforasignificantlymorespecificantibodydetectionthanconventionalmethodsbasedonnativeantigens.
Evaluationofdiagnosticperformance
Theassessmentofdiagnosticsensitivityandspecificitywascarriedoutusingconfirmedpositivespecimens(CerbaSpecimenServicesandINFORMM,Penang,Malaysia)aswellasnegativesamples(bloodbank)andserathatarepotentiallycross-reactive.
48Strongyloidesantibodynegativesamples
33Strongyloidesantibodypositivesamples
| Confirmedsamples | |||
| Positive | Negative | ||
| StrongyloidesAbELISA | Positive | 29 | 2 |
| Negative | 4 | 46 | |
OurStrongyloidesAbELISAboastsadiagnosticsensitivityof87.9%andspecificityof95.8%,andthereforefulfillstherequirementsofaneffectivediagnosticmethodforstrongyloidiasis.
