| Kitsize | 100tests(3mL) |
| Method | Reagent |
| Incubationtime | No |
| Standardrange | No |
| Specimen/Volumes | No |
| Substrate/isotope | No |
| RegulatoryStatus: | EU:CE |
SummaryandExplanation:
RheumatoidfactorsareautoantibodiesmainlyoftheIgM-class.TheybindpreferablytoIgGantibodies.AmongtheunspecificIgGantibodiesinpatientsamplethereareoftenspecificIgGsdirectedagainstthecoatedantigen.TheRheumatoidfactorIgMantibodiesbindtothesespecificIgGantibodieswhicharedirectedagainsttheantigencoatedtotheplate.Thiscomplexisdetectedbytheantihumandetectionantibodies.Thisleadstofalsepositiveresultsinthepathogen-specificIgM-detection.
Anotherproblemthatoccursinthepathogen-specificIgM-detectionisthedisplacementofweakbindingpathogenspecificIgMantibodiesbystrongerbindingIgGantibodies.Thisleadstofalsenegativeresultsinthepathogen-specificIgM-detection.TheantihumanIgGantibodiesintheRF-AbsorbentbindtotheIgGantibodiesinthesample.AcrosslinkingtakesplacebetweentheIgGantibodies,theIgGantibodiesboundtotheRheumatoidfactorIgMantibodiesandtheantihumanIgGantibodies.Inthefollowingaprecipitationofthecomplextakesplace.
Thisprecipitationhastwogoals:
- TheeliminationofspecificIgGantibodiesdirectedagainsttheantigencoatedtotheplate.(Avoidanceoffalsenegativeresult)
- TheeliminationoftheRheumatoidfactorIgMantibodieswhichareboundtothespecificIgGantibodiesinthesample.(Avoidanceoffalsepositiveresults)
ThereforeIBLInternationalstronglyrecommendsthepretreatmentofpatientsamplesinthepathogen-specificIgM-detection.
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