IBL/肾素活性发光免疫分析/DB69011/
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产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Kitsize | 12x8 |
Method | LuminescenceImmunoassay |
Incubationtime | 1x1h,1x30min |
Standardrange | 0.2-60ng/mL |
Specimen/Volumes | 500µLEDTAPasma |
Substrate/isotope | Luminol |
RegulatoryStatus: | RUO,EU:CE |
Detailsfor: ReninactivityLuminescenceImmunoassay
LuminescenceImmunoassayforthequantitativedeterminationofPlasmaReninActivity(PRA)inhumanplasma.
MeasurementofPRAisimportantfortheclinicalevaluationofhypertensivepatients.Inparticular,determinationofplasmareninactivitycanhelpinthediagnosisofprimaryhyperaldosteronism(5-13%ofhypertensivecases)andassistinthetherapyandmanagementofotherformsofhypertension.
PRA,incontrasttothedeterminationofreninconcentration,isamoreaccurateindicatorofprimaryhyperaldosteronism(PHA),becauseofseveralreasons:
1.PRAistheexpressionoftherateofAng-Iformationthroughtheenzymaticactionofreninonitssubstrate,angiotensinogen,thereforePRAdependsnotonlyonreninconcentrationbutalsoontheconcentrationofangiotensinogenwhichisignoredinthereninconcentrationassay;
2.Plasmareninconcentrationassaydoesnotensuresensitivityinlowreninstates,whilethesensitivityofthePRAassaycanbeenhancedbyincreasingtheincubationtimeduringthegenerationstep(Sealeyetal.,2005),
3.WhenaninhibitorisboundtothereninactivesitePRAisinhibited,whereasthepresenceoftheinhibitordoesnotaffecttherecognitionofreninbycurrentlyavailableimmunoassays,thereforetotalreninconcentrationdoesnotalwayscorrelatewithplasmareninactivity(Campbelletal.,2009).
Reninliberatesangiotensin-Ifromangiotensinogen.Angiotensin-Iistransformedtoangiotensin-IIlargelyinpulmonarycirculationbyangiotensinconvertingenzyme(ACE).Angiotensin-IIraisesbloodpressurebydirectarteriolarvasoconstriction,promotingsodiumretension,andstimulatingthesecretionofaldosteronefromtheadrenalcortex.Aldosteronealsoexertsaneffecttorestoresodiumbalanceandliftarterialpressure.Accuratemeasurementoftheconcentrationofcirculatingangiotensin-IIischallengingbecauseofitsinstABIlityinbloodsamples.Aldosteroneconcentrationcanbeeasilydeterminedusingtheimmunoassaykit.
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.MeasurementofPRAisimportantfortheclinicalevaluationofhypertensivepatients.Inparticular,determinationofplasmareninactivitycanhelpinthediagnosisofprimaryhyperaldosteronism(5-13%ofhypertensivecases)andassistinthetherapyandmanagementofotherformsofhypertension.
PRA,incontrasttothedeterminationofreninconcentration,isamoreaccurateindicatorofprimaryhyperaldosteronism(PHA),becauseofseveralreasons:
1.PRAistheexpressionoftherateofAng-Iformationthroughtheenzymaticactionofreninonitssubstrate,angiotensinogen,thereforePRAdependsnotonlyonreninconcentrationbutalsoontheconcentrationofangiotensinogenwhichisignoredinthereninconcentrationassay;
2.Plasmareninconcentrationassaydoesnotensuresensitivityinlowreninstates,whilethesensitivityofthePRAassaycanbeenhancedbyincreasingtheincubationtimeduringthegenerationstep(Sealeyetal.,2005),
3.WhenaninhibitorisboundtothereninactivesitePRAisinhibited,whereasthepresenceoftheinhibitordoesnotaffecttherecognitionofreninbycurrentlyavailableimmunoassays,thereforetotalreninconcentrationdoesnotalwayscorrelatewithplasmareninactivity(Campbelletal.,2009).
Reninliberatesangiotensin-Ifromangiotensinogen.Angiotensin-Iistransformedtoangiotensin-IIlargelyinpulmonarycirculationbyangiotensinconvertingenzyme(ACE).Angiotensin-IIraisesbloodpressurebydirectarteriolarvasoconstriction,promotingsodiumretension,andstimulatingthesecretionofaldosteronefromtheadrenalcortex.Aldosteronealsoexertsaneffecttorestoresodiumbalanceandliftarterialpressure.Accuratemeasurementoftheconcentrationofcirculatingangiotensin-IIischallengingbecauseofitsinstABIlityinbloodsamples.Aldosteroneconcentrationcanbeeasilydeterminedusingtheimmunoassaykit.
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