Original article #27180
HTGL (hepatic triacylglycerol lipase / hepatic triglyceride lipase) is a secreted glycoprotein and known as a lipolytic enzyme and is also called hepatic lipase (HL). HTGL has an important role in lipoprotein metabolism as a lipolytic enzyme that hydrolyzes triglycerides (TG) and phospholipids in chylomicron remnants, intermediate-density lipoproteins (IDL) and high-density lipoproteins (HDL). It has been reported that hypercholesterolemia or triglyceridemia is observed and very-low-density lipoproteins (VLDL), chylomicron remnants, IDL, TG-rich low-density lipoproteins (LDL) and HDL are accumulated in patients with deficiency of HTGL. HTGL is synthesized by hepatocytes and bound to heparin sulfate proteoglycans at the surface of liver sinusoidal capillaries. In general speaking, it is essential for use heparin plasma that is collected after intravenous injection of heparin (postheparin plasma) for measurement of HTGL in blood sample as same as lipoprotein lipase (LPL) as HTGL that bonds and adheres to surface of endothelial cells is removed from capillary vascular bed and released into the blood stream soon after heparin injection. This ELISA kit can detect HTGL in serum and plasma regardless of administration of heparin that is conventionally required for the measurement.