IBL/骨桥蛋白N半ELISA/JP27258/
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h,2x30min |
| Standardrange | 6.25-400pmol/L |
| Specimen/Volumes | 100µLplasma,urine,synovialfluid,cellculturesupernatant |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | Forresearchuseonly |
Detailsfor:OsteopontinN-HalfELISA
Osteopontin(OPN)isknownasahighlyacidiccalcium-bindingglycosylatedphosphoproteinsecretedbymanycelltypes,includingosteoblasts,kidneytubulecells,macrophages,activatedTcells,andvascularsmoothmusclecells.Itsmolecularweightshavebeenreportedintherangeof66kDato44kDadependingonglycosylationandphosphorylation.OneimportantfeatureofOPNisthatitcontainsanArg-Gly-Asp(RGD)aminoacidsequence.Thismotifispresentinfibronectin,vitronectinandavarietyofotherextracellularproteinsthatbindmembersoftheintegrinfamilyofcellsurfacereceptorssuchasavß3.AnotherimportantpointisthepresenceofvariousmolecularformsofOPNinvivoduetodifferentialRNAsplicing,glycosylation,phosphorylation,sulfation,andsusceptibilitytoproteases.BothOPNandthrombinarelikelytobelocalizedtogetheratthesiteofinjury,inflammation,andangiogenesisandintumortissues.OsteopontinissusceptIBLetoproteolyticfragmentation,andthisprocessmayhavephysiologicimportance.ThekitcanspecificallyassayforN-terminalfragmentofthrombin-cleavedhumanOPN(OPNN-Half),andalmostcannotdetectuncleavedOPN.
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.品牌介绍
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