| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 2x1h,1x30min |
| Standardrange | 2-200U/ml |
| Specimen/Volumes | 10µlserum,plasma |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE,CDN:IVD |
Measles-
AThreatStillMuchAlive
IBLInternationaloffershighlysensitiveandspecificELISAsfortheSEROlogicaldiagnosisofacute-phasemeaslesvirusinfection(IgM)aswellasforthedeterminationoftheimmunestatus(IgG),i.e.asanindicatorofsuccessfulmeaslesvaccinationoraftermeaslesvirusexposure.Theyhavefollowingfeatures:
- μ-capturetechnologyforIgM
- IgMELISA:diagnosticsensitivityof96%/diagnosticspecificityof99%
- IgGELISA:diagnosticsensitivityof99%/diagnosticspecificityof96%
- CalibratedagainsttheWHOstandard(IgG)
- Quantitative(immunestatus)or,alternatively,qualitativeresults
- Nocross-reactivitywithmumps,rubellaorEBV
- ReADIlyadaptableforuseinopenautomatedELISAsystems
- Kitshelflifeof18months
About30millionpeopleworldwidecontractmeasleseveryyear.Althoughaneffectiveandaffordablevaccinehasbeenavailablefor40years,measlesisstilloneoftheleadingcausesofdeath,especiallyinchildren.Althoughthenumberofmeaslescasesinyoungchildrenhadgreatlydiminishedduetosystematicvaccinationcampaigns,asignificantre-emergenceofmeasleshasagainbeenobservedwithinthepast10years.Lastyear,large-scaleoutbreaksoccurredinAsia,Africa,SouthAmericaandEurope.Themeaslesvirusistransmittedbydirectcontactwithnasalorpharyngealsecretionsandbydropletroute.Itishighlycontagious--virtuallyallcontactsoftheinfectedpersonwillcontractthedisease.Symptomsrangefromflu-likesymptomsaccompaniedbyacharacteristicrashtoseverecomplicationswithneurologicaldeficitsandorganfailure.
Inthepast,measlesinfectionposednodiagnosticchallengeduetoitstypicalclinicalpicture.Aftertheintroductionofvaccination,however,thesymptomshavebecomerare,sothatlaboratorydiagnosisisbecomingincreasinglyimportant.SerologicaldetectionofIgMantibodiesisthefastestandmostconclusivemethod.IgMantibodiestypicallyemergewiththeappearanceoftherash.
AssessmentofthediagnosticperformanceofmeaslesIgMμ-captureELISAandmeaslesIgGELISA
1.Methodcomparison:IBLInternational"sMeaslesIgMμ-captureELISAvs.SiemensEnzygnostAnti-MeaslesIgMELISA(n=204)
| IBLInternational | |||||
| positive | borderline | negative | |||
| positive | 44 | 1 | 1 | 46 | |
| Siemens | borderline | 3 | 2 | 6 | 11 |
| negative | 1 | 1 | 145 | 147 | |
| 48 | 4 | 152 | 204 | ||
Diagnosticsensitivity:95.7%
Diagnosticspecificity:98.6%
2.Methodcomparison:IBLInternational"sMeaslesIgGELISAvs.SiemensEnzygnostAnti-MeaslesIgGELISA(n=258)
| IBLInternational | |||||
| positive | borderline | negative | |||
| positive | 170 | 2 | 0 | 172 | |
| Siemens | borderline | 0 | 10 | 0 | 10 |
| negative | 1 | 2 | 73 | 76 | |
| 171 | 14 | 73 | 258 | ||
Diagnosticsensitivity:98.8%
Diagnosticspecificity:96.1%
GeneralInformation
Measlesisahighlycontagiousviraldiseasecharacterizedbyaclinicallydistinctprodromeoffever,coryza,conjunctivitis,coughandapathognomicexanthem(Koplik`sspots).TherashofMeaslesappearsaftera3to5-daysprodrome,some14daysafterexposureandmaybeassociatedwithedemaoftheskin.ThediseaseistheresultofinfectionwiththeMeaslesVirus,genusMorbillivirusofthefamilyParamyxoviridae.Complicationsare:otitismedia,pneumoniaandencephalitis.MeasleshaveamoresevereexpressioninyoungerorundernourishedchildrenwithahigherincidenceofhemorrhageMeasles,with5%to10%oflethalcases.MeaslesareoneofthemostcontagiousInfectiousDiseases.Thevirusspreadsthroughdropletsemanatingfromtherespiratorytractofinfectedpersonsorbydirectcontact.TheincidenceofMeasleshasdeclinedsincetheintroductionofvaccinationprograms.
