| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h,1x30min,1x15min |
| Standardrange | cut-offindex |
| Specimen/Volumes | 10µLserum,plasma |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
Bacteriafromraturine
Forthepurposeofdiagnosingleptospirosis,themostcommonzoonoticdisease,IBLInternationalnowoffersanewhighlysensitiveandspecificELISAforthedetectionofIgMandIgGantibodies:
- Diagnosticsensitivity>99%;diagnosticspecificity>96%
- Indication:clinicalsUSPicionofleptospirosis/Weil"sdisease
- DetectionoftheimmuneresponseagainstallLeptospiraSEROtypes
- Qualitativeresults(positive,negative,cut-offcontrol)
- Adaptabletoopen-systemELISAanalyzers
LeptospirosisisanacutetochroniczoonoticdiseasecausedbypathogenicspeciesofLeptospira.Thesourcesofinfectionarerodents,mainlyratsandmice.Theanimalsexcretethepathogensintheurine.Theycanenterthehumanbodythroughsmallcutsintheskin.Thenumberofnewcasesworldwideisestimatedat300,000to500,000cases.Majoroutbreakshavebeenobservedinparticularinthesettingofflooding.Intheindustrializednations,localoutbreaksoccurprimarilyinthecontextofrecreationalactivitiessuchaswatersportsorboattrips.Approximately90%ofpatientshavemildflu-likesymptoms.Theseverecourseofdisease,alsoknownasWeil"sdisease,ischaracterizedbybleeding,renalfailure,jaundiceandfatalcomplications.
Pathogenscanberecoveredfrombloodculturesonlyinthefirstphaseofthedisease.Theculturetakesquitealongtimeaswell,meaningthatasuccessfulantibiotictreatmentcannotbeinstitutedinatimelymanner.Forthisreason,serologicalmethodsareincreasinglyused.Theserologicalreferencemethodisthemicroagglutinationtest,which,however,istime-consumingandcarriesariskofinfectionbecauseitemployslivecultures.ThisaccountsforthespecialsignificanceoftheELISAmethodforthedeterminationofspecificantibodies,whichcanbedetectedwithin5-7daysaftertheonsetofclinicalsymptoms.ThepresenceofspecificIgMantibodiesisconclusiveforacuteillness.
EvaluationofthediagnosticperformanceofLeptospiraIgMELISA
MethodcomparisonofIBLInternationalLeptospiraIgMELISAwiththeLeptospiraIgMclassicELISA(Serion)
Thecomparisonof37positiveandnegativeseraresultedinanagreementof96.7%.
Serion positive negative IBLInternational positive 5 1 negative 0 24
EvaluationofthediagnosticperformanceofLeptospiraIgGELISA
MethodcomparisonofIBLInternationalLeptospiraIgGELISAwiththeLeptospiraIgGclassicELISA(Serion)
Thecomparisonof45positiveandnegativeseraresultedinanagreementof97.6%.
| Serion | |||
| positive | negative | ||
| IBLInternational | positive | 4 | 1 |
| negative | 0 | 37 | |
