| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h,1x30min,1x20min |
| Standardrange | 0-150IU/mL |
| Specimen/Volumes | 5µLserum,plasma |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
Pertussis,alsoknownaswhoopingcough,isarespiratoryinfectionthatcausesapersistentcoughthatmayaffectallagegroups.
TheserologyofapertussisinfectionissignificantlyimprovedbythedetectionofantibodiesdirectedagainstmoleculesthatareproducedbyBordetellabacteria,likepertussistoxin(PT)andfilamentoushaemagglutinin(FHA).
| BioMarker | ||
| PT | FHA | |
| Bordetellapertussis | + | - |
| Bordetellaparapertussis | + | + |
Biomarkerforthepertussisserologie
Fortheserologyofanacutepertussisinfection,itisgenerallysufficienttomeasureIgGantibodiesagainstPT.ItallowsacleardifferentiationofaninfectionwithBordetellapertussisfromBordetellaparapertussisandisusefultoassesstheimmunostatusaftervaccination.Theresultsareinterpretedaccordingtothefollowingscheme.
| Pertussistoxinlevels(IU/mL) | Interpretation |
| <40 | Noacuteinfection;furthertestsonlynecessarywhencorrespondingsymptomsarepresent |
| 40-100 | Unclear;repeatthetestseveraldayslater |
| >100 | Acuteinfectionorrecentvaccination |
Interpretationofresults
DirectmethodcomparisonshowssimilardiagnosticspecificityandsensitivitybetweentheIBLInternationalBordetellaPTIgGELISAandacommerciallyavailableBordetellaPTIgGELISA.
| CommercialBordetellaPTIgGELISA | ||||||||||||||||
| IU/mL | <40 | 40-100 | >100 | |||||||||||||
| IBLInternationalBordetellaPTIgGELISA |
| |||||||||||||||
Methodcomparison
Literature
TrollforsB.etal.DeterminationofpertactinIgGantibodiesforthediagnosisofpertussis.ClinMicrobiolInfect2003;9:585–589.
WatanabeM.etal.CharacterizationofSerologicalResponsestoPertussis.ClinVaccineImmunolMarch2006vol.13no.3341-348.
AmirthalingamG.etal.GuidelinesforthePublicHealthManagementofPertussis.www.hpa.org.uk.February2011.
ExcerptfromtheInstructionsforUse
Pertussis,alsoknownaswhoopingcough,isarespiratoryinfectionthatcausesapersistentcoughinallagegroupsfromthenewborntotheelderly.BordetellapertussisbacteriaproduceaseriesofBIOLOGicallyactivemoleculeslikepertussistoxin(PT),filamentoushaemagglutinin(FHA)andvariouslipopolysaccharides(LPS).Thesevariouscompoundsappeareitherduringthepathogenesisorduringtheprocessofimmunization.PTisspecificforbordetellapertussiswhereasFHAisfoundinbordetellapertussisandparapertussis.NearlyallacellularvaccineslicensedinEuropecontainPTandFHAinsubstantialamounts.Over90%ofresponsesarethereforedirectedagainsttheimmunogensPTandFHA.
TodiagnoseanacuteBordetellapertussisinfectionserologically,itis,accordingtoup-to-dateknowledge,usuallysufficienttoperformasingledeterminationofIgGantibodiesagainstpertussistoxin(PT).Thesecondbloodwithdrawalisomitted,andresultsareinterpretedaccordingtothefollowingscheme:
<40IU/mL-Noindicationofanacuteinfection;furthertestsonlynecessarywhencorrespondingsymptomsarepresent
40-100IU/mL-Unclearserologicalconstellation;investigateasecondsampleseveraldayslater.
>100IU/mL-Indicationofanacuteinfectionorrecentvaccination.
