ThenewIBLInternationalProgesteroneSalivaELISAiscalibratedtotheLC-MS/MSreferencemethodandtotheluminescenceImmunoassay(IBLProgesteroneSalivaLUM,RE62021).

Thisnewassayrequiresaslowas50µLSalivasamplevolumeandhasabroadstandardrangeupto5,000pg/mL.

BenefitofStandardizedreagents

OurSalivaELISAproductlinehasbeenrevisedsothatthemainsteroids(cortisol,testosterone,progesterone,DHEA)maybeprocessedinasimilarway:

• Thesubstrate,washingandstopsolutionsareinterchangeable;
• Theenzymeconjugate,thesubstrateandstopsolutionsareuniformlyPipettedwith100µL;
• Thekitscontainasfewas6standardsand2controls;
• Only50µLsamplevolumeisrequired;
• Allkitsusethesamesamplediluentbuffer.

Thankstothesimilarprocessingandassembling,theassayscaneasilybeper-formedincombination.

Applicationfields

ThetwomainindicationsforprogesteronemeasurementsaretheCorpusLuteuminsufficiencyaswellastheanovulatoricalmenstrualcyclesinfemales.Inanti-agingmedicineitmightbenecessarytoevaluatetheProgesteronelevelbeforeandduringthereplacementtherapy.

Pulsatiledynamics

Duetothepulsatiledynamicsofsteroidsecretionrepeatedsalivasamplingisrec-ommended.IBLrecommendscollecting3to5salivasampleswithin2hours.Inthelaboratory,equalvolumesoftheindividualsalivasamplescanbemixed.ThismixedsampleresultsinameanfreeProgesteronevalue,whichrepresentstheactivehormoneconcentrationinareproducibleway.

InformationfromtheInstructionsforUse

Measurementsobtainedbythisassaymaybeusedinthediagnosisandtreatmentofdisordersoftheovariesorplacentaandcanbeusedasanaidforpredictionofovulation.TheProgesteronelevelinsalivarepresentstheconcentrationoftheactivefreeProgesterone.

Progesterone(4-pregnene-3,20-dione)isaC21steroidhormonecontainingaketo-group(atC-3)andadoublebondbetweenC-4andC-5.Likeothersteroids,itissynthesizedfromcholesterolviaaseriesofenzyme-mediatedsteps.ThesteroidhormoneProgesteroneisafemalesexhormonewhich,inconjunctionwithestro-gens,regulatestheaccessoryorgansduringthemenstrualcycleanditisparticu-larlyimportantinpreparingtheendometriumfortheimplantationoftheblastocyteandinmaintainingpregnancy.Innon-pregnantwomenprogesteroneismainlysecretedbythecorpusluteumwhereasinpregnancytheplacentabecomesthemajorsource.Minorsourcesforprogesteronearetheadrenalcortexforbothsexesandthetestesformales.