IBL/PLGF-ELISA/RE52361/
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h,3x30min |
| Standardrange | 25-1000pg/mL |
| Specimen/Volumes | 25µLserum |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
Detailsfor:PLGFELISA
Enzymeimmunoassayforthequantitativedeterminationofhumanplacentagrowthfactor(PLGF)inhumanserum.
PRINCIPLEOFTHETEST
ThePLGFELISAKitisasolidphaseenzyme-linkedimmunosorbentassay(ELISA)basedonthesandwichprinciple.
ThemicrotiterwellsarecoatedwithamonoclonalantibodydirectedtowardsauniqueantigenicsiteofthePLGFmolecule.AnaliquotofpatientsamplecontainingendogenousPLGFisincubatedinthecoatedwell.
Afterawashingstepabiotin-linkedpolyclonalantibodyspecificforPLGFisaddedtothewells.FollowingawashtoremoveanyunboundantibodyastreptavidinHRPenzymecomplexisaddedtothewells.Afterincubationtheunboundenzymecomplexiswashedoff.TheamountofboundperoxidaseisproportionaltotheconcentrationofPLGFinthesample.Havingaddedthesubstratesolution,theintensityofcolourdevelopedisproportionaltotheconcentrationofPLGFinthepatientsample.
SummaryandExplanation
Agiogenesisandvasculartransformationareimportantprocessesinthenormaldevelopmentoftheplacenta.Abnormalangiogenesisandvasculartransformationareconsideredtobeoneofthemainreasonsforpreeclampticpregnanciesandintrauterinegrowthretardation.PlacentalgrowthfactorPLGF,amemberoftheVEGFfamily,isproducedmainlybytheplacentaandisapotentangiogenicfactor.Thecorrespondingreceptor,thesolublefms-liketyrosinekinase-1isconsideredtohaveangiogenicproperties.
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.PRINCIPLEOFTHETEST
ThePLGFELISAKitisasolidphaseenzyme-linkedimmunosorbentassay(ELISA)basedonthesandwichprinciple.
ThemicrotiterwellsarecoatedwithamonoclonalantibodydirectedtowardsauniqueantigenicsiteofthePLGFmolecule.AnaliquotofpatientsamplecontainingendogenousPLGFisincubatedinthecoatedwell.
Afterawashingstepabiotin-linkedpolyclonalantibodyspecificforPLGFisaddedtothewells.FollowingawashtoremoveanyunboundantibodyastreptavidinHRPenzymecomplexisaddedtothewells.Afterincubationtheunboundenzymecomplexiswashedoff.TheamountofboundperoxidaseisproportionaltotheconcentrationofPLGFinthesample.Havingaddedthesubstratesolution,theintensityofcolourdevelopedisproportionaltotheconcentrationofPLGFinthepatientsample.
SummaryandExplanation
Agiogenesisandvasculartransformationareimportantprocessesinthenormaldevelopmentoftheplacenta.Abnormalangiogenesisandvasculartransformationareconsideredtobeoneofthemainreasonsforpreeclampticpregnanciesandintrauterinegrowthretardation.PlacentalgrowthfactorPLGF,amemberoftheVEGFfamily,isproducedmainlybytheplacentaandisapotentangiogenicfactor.Thecorrespondingreceptor,thesolublefms-liketyrosinekinase-1isconsideredtohaveangiogenicproperties.
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