| Kitsize | 2x(12x8) |
| Method | ELISA |
| Incubationtime | 1x1h,1x2h,2x30min |
| Standardrange | MN:0-2500pg/mL;NMN:0-3000pg/mL |
| Specimen/Volumes | 200µlEDTAPlasma |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
Enzymeimmunoassayforthequantitativedeterminationoffreemetanephrineandnormetanephrineinhumanplasma.
Catecholaminesandtheirmetabolites,metanephrineandnormetanephrine,aresecretedinexcessiveamountbyvarioustumors,includingparaganglioma,neuroblastoma,andinparticularpheochromocytoma.TheclinicalmanifestationofthesetumorsishighlyvariableandoftenresemblessymptomsassociatedwithcommonmalADIes,predominantlyhypertension,headacheandsweating.
Biochemicaltestingforpheochromocytomaisindicatednotonlyinsymptomaticpatients,butalsoinpatientswithadrenalincidentalomasoridentifiedgeneticpredispositions.Itinvolvesmeasurementsofcatecholamines(adrenaline,noradrenalineanddopamine)andtheirmetabolites(metanephrine,normetanephrineandvanillylmandelicacid)inplasmaorurine.
NumerousstudieshaveshownthatfreemetanephrinesinplasmaarethebestMarkersforthediagnosisofpheochromocytomes(Grossmannetal,2006;Lendersetal.,2005;Eisenhoferetal.,2000)
Sensitivity Specificity Plasma–freemetanephrines 99% 89% Urinary–fractionatedmetanephrines 97% 69% Urinarycatecholamines 86% 88% Plasmacatecholamines 84% 83% Totalurinarymetanephrines 77% 93% Vanillylmandelicacid(VMA) 64% 95%
Lendersetal.Lancet(2005)
IBLInternational’sMetCombiPlasmaELISAhasbeenimproved
- Shorterassaytime:ca.4.5h
- CalibratedtoLC–MS/MS
- Automatedtestprocedure(DSX)
- Smallersamplevolume:200µL
- Highersensitivity:
Metanephrine:7pg⁄mL
Normetanephrine:7pg/mL
IBLInternationalMetCombiPlasmaELISAcorrelateswelltoLC–MS⁄MS.
ThemanualandautomatedperformancesofIBLInternationalMetCombiELISAcorrelatewell.
ExcerptfromtheInstructionsforUse
Enzymeimmunoassayforthequantitativedeterminationoffreemetanephrineandnormetanephrineinhumanplasma.Thecatecholaminesadrenalin,noradrenalinanddopaminearesynthesizedintheadrenalmedulla,thesympatheticnervoussystemandinthebrain.Theyinfluencevirtuallyalltissuesandareinvolvedtogetherwithotherhormonalandneuronalsystemsintheregulationofawidevarietyofphysiologicalprocesses.
Ascatecholaminesandtheirmetabolitesmetanephrineandnormetanephrinearesecretedinincreasingamountsinanumberofdiseases,theymaybeusedfordiagnosticpurposes.
Inthiscontext,diagnosisaswellasthefollow-upoftumordiseasesofthenervoussystemareofspecialimportance.Thisappliesprimarilytothepheochromocytoma,butalsotheneuroblastomaandtheganglioneuroma.
Malignantgrowthisdescribedin10%ofpheochromocytomas.FurThermore,anincreaseofcatecholaminesandtheirmetabolitesmetanephrineandnormetanephrinecanbeobservedinthecarcinoid.
Dataofthelastyearshaveshownthatthemetanephrinesinplasmaarethebestmarkerfordiagnosisandfollow-upofpheochromocytoma.
