| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x90min.,1x30min.,1x15min. |
| Standardrange | 0-130ng/mL |
| Specimen/Volumes | 25µLserum |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
TheroleofvitaminDindevelopmentofbone-relateddiseasesintheboneandmineralmetabolismhadbeendiscoveredsincemanyyears.
Clinicalrelevance
Theaccurateassessmentofaperson’svitaminDstatusreliesonthemeasurementof25-OH-vitaminDtotal(25-OH-vitaminD2+25-OH-vitaminD3)levels.ThereforeourassayhasbeendevelopedtodetectbothD2andD3variants.TheroleofvitaminDinregulatingthecalcium-phosphorusbalancefornormalbonemineralizationiswellknownsincemanyyears.Thusthedeterminationof25-OH-vitaminDhaslongbeenusedfordiagnosisandmonitoringtherapyofrickets,renalosteodystrophy,osteomalaciaandpostmenopausalosteoporosis.
Morerecentstudiesindicatethatinsufficient(seetablebelow)vitaminDlevelsarecorrelatedwithincreasedriskofdevelopingotherdiseases,suchascancer,hypertension,diabetes,cardiovasculardiseasesandmanymore.
Suggestedreferencevalues Deficiency <10ng/mL Insufficiency 10-30ng/mL Sufficiency 30-100ng/mL Toxicity >100ng/mL
CorrelationtoLC-MS/MS
TheIBL-International25-OH-VitaminDELISAiscorrelatedagainsttheLC-MS/MSreferencemethod.
Thecorrelationwasperformedon135serumsamplescomparingtheIBL-International25-OH-VitaminDELISAtotheLC-MS/MS.Theregressionanalysisshowedaslopeof1.042,aninterceptof-3.731ng/mLwithacorrelationofR=0.92.
Automation
TheIBLInternational25-OH-VitaminDELISAhasbeendevelopedtobeadaptabletoopenELISAprocessors.Anin-housecorrelationhasbeenperformedcomparingthemanualwork-upofthe25-OH-VitaminDELISAandtheautomatedwork-upofthe25-OH-VitaminDELISAontheTECANFreedomEVOlyzer®.Theregressionanalysisshowedaslopeof0.9737,aninterceptof-0.269ng/mLwithacorrelationofR=0.99.
AdvantagesofourELISA
- ExcellentcorrelationtotheLC-MS/MSreferencemethod
RE53041=1.042xLCMS-3.731,R=0.92 - Directassaywithoutextraction
- Ready-to-usereagents
- EasilyadaptableonopenELISAplatforms
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