IBL/血管紧张素I（PRA）RIA（CT）/30120823/
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Kitsize | 100 |
| Method | RIA(CT) |
| Incubationtime | 1x1h,1x2h |
| Standardrange | 0-30ng/mL |
| Specimen/Volumes | 200µLEDTAPlasma |
| Substrate/isotope | 125-I |
| RegulatoryStatus: | EU:CE |
Detailsfor:AngiotensinI(PRA)RIA(CT)
RADIoimmunoassayofAngiotensinIforthedeterminationofPlasmaReninActivity(PRA)inhumanplasma.Forin-vitrodiagnosticuseonly.Forprofessionaluseonly.
PRINCIPLEOFTHEASSAY
TheAngiotensinIRIAservesforthequantitativedeterminationofplasmareninactivity(PRA)bytheradioimmunoassayoftheproductofthereaction,angiotensinI.
ThegenerationofangiotensinIistheresultoftheenzymaticcleavageofthereninsubstrate,angiotensinogen,inplasmasamplesinthepresenceofACEinhibitor(ACE-Angiotensin-ConvertingEnzyme),anenzymaticinhibitorthatblockstheconversionofangiotensinItoangiotensinII.TheimmunoassayofangiotensinIisaradioimmunologicalcompetitionassay.Unknownsamples,control,andcalibratorsareincubatedinpolyclonalantibody-coatedtubeswith125I-labeledangiotensinIastracer.Afterincubation,thecontentsofthetubesareaspirated.Theboundradioactivityisthendeterminedinagammacounter.Astandardcurveisestablishedandunknownvaluesaredeterminedbyinterpolationfromthestandardcurve.
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.PRINCIPLEOFTHEASSAY
TheAngiotensinIRIAservesforthequantitativedeterminationofplasmareninactivity(PRA)bytheradioimmunoassayoftheproductofthereaction,angiotensinI.
ThegenerationofangiotensinIistheresultoftheenzymaticcleavageofthereninsubstrate,angiotensinogen,inplasmasamplesinthepresenceofACEinhibitor(ACE-Angiotensin-ConvertingEnzyme),anenzymaticinhibitorthatblockstheconversionofangiotensinItoangiotensinII.TheimmunoassayofangiotensinIisaradioimmunologicalcompetitionassay.Unknownsamples,control,andcalibratorsareincubatedinpolyclonalantibody-coatedtubeswith125I-labeledangiotensinIastracer.Afterincubation,thecontentsofthetubesareaspirated.Theboundradioactivityisthendeterminedinagammacounter.Astandardcurveisestablishedandunknownvaluesaredeterminedbyinterpolationfromthestandardcurve.
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