IBL/5-HIAA ELISA（5-羟基-3-吲哚乙酸）/RE59131/
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产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x18h,1x2h,1x1h |
| Standardrange | 0.4-55mg/L |
| Specimen/Volumes | 20µLurine |
| Substrate/isotope | PNPP405nm |
| RegulatoryStatus: | EU:CE,CDN:IVD |
Detailsfor:5-HIAAELISA(5-Hydroxy-3-indoleaceticacid)
Enzymeimmunoassayforthein-vitro-diagnosticquantitativedeterminationof5-HIAAinhumanurine.Theprimarycarcinoidtumorisusuallyderivedfromtheenterochromaffincellsofthemidgutandislocatedmostfrequentlyintheterminalileum.Carcinoidtumoursgenerallysecretevariousamountsofindoles.Thecarcinoidsyndromeisgenerallycharacterizedbyanincreasedurinaryexcretionof5-hydroxy-3-indoleaceticacid(5-HIAA),theendproductofSEROtonin(5-HT)metabolism.
TrADItionally,5-hydroxy-3-indoleaceticacidisassayedbydiazotizationwithnitrosonaphtoltoformapurplecolour.However,itiswelldocumentedthatmanyothersubstancespresentintheurineinterferewiththisreactiontogivefalse-positiveresults.Attemptsweremadetoovercomethisproblembyacom¬binationofionexchangechromatographyandfluorometry.Thesemethods,however,lacksensitivityandaretimeconsuming.Recently,highperformanceliquidchromatographicanalysesof5-HIAAwithfluorometryintheultravioletregionofthespectrumorelectRochemicaldetectionhavebeendescribed.Bothmethodsrequiresolventextractionbecauseofthenumerousinterferingcompoundspresentinurine.The5-HIAAenzymeimmunoassayisanewandsimplemethodforthequantificationofthisimportantMarkerofcarcinoidsyndromeinsmallurinesamples.
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.TrADItionally,5-hydroxy-3-indoleaceticacidisassayedbydiazotizationwithnitrosonaphtoltoformapurplecolour.However,itiswelldocumentedthatmanyothersubstancespresentintheurineinterferewiththisreactiontogivefalse-positiveresults.Attemptsweremadetoovercomethisproblembyacom¬binationofionexchangechromatographyandfluorometry.Thesemethods,however,lacksensitivityandaretimeconsuming.Recently,highperformanceliquidchromatographicanalysesof5-HIAAwithfluorometryintheultravioletregionofthespectrumorelectRochemicaldetectionhavebeendescribed.Bothmethodsrequiresolventextractionbecauseofthenumerousinterferingcompoundspresentinurine.The5-HIAAenzymeimmunoassayisanewandsimplemethodforthequantificationofthisimportantMarkerofcarcinoidsyndromeinsmallurinesamples.
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