| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x2h,1x30min |
| Standardrange | 1.7-420ng/mL |
| Specimen/Volumes | 10µLurine |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE,CDN:IVD |
Melatonin–Morethanasleephormone
IBLInternationalistheonlycommercialsupplierofacompletesetofuserfriendlyimmunoassaysforthereliablequantitativeassessmentofmelatonininserumaswellasinsaliva,andofitsprimarymetabolite6-sulfatoxymelatonininurine.
- TheassaysarecalibratedtoLC-MS/MS
- Thebroadstandardrangeallowsapplicationfortherapeuticdrugmonitoring
- Validatedreferencevaluesforurinary6-sulfatoxymelatonin(figure1)1
- Successfullyappliedindietresearch2-4,variousspeciesandcellculturesupernatants*
- Widelyusedandcitedinmultiplepublications*
Timeandagedependentreferencevaluesofurinary6-sulfatoxymelatoninmeasuredwithIBLInternational’sMelatonin-SulfateUrineELISA1
Melatonin:aubiquitouslydistributedandfunctionallydiversemolecule
Melatoninisanendogenoushormonesynthesizedandsecretedprimarilybythepinealgland.Itssecretionisregulatedbythesuprachiasmaticnucleiandregulatesthediurnallight-darkcyclewithpeakproductionatnightandlowlevelduringthedaytime.
Apartfromitsroleintheregulationofsleep,multiplestudiesshowitsinvolvementinawidevarietyofphysiologicalprocesses(affectingcircADIanrhythmandmood,interactingwiththeimmunesystemandactingasantioxidant,freeradicalscavengerandoncostaticagent).Moreover,ithasbeenshownthatmelatoninperformsthesefunctionsbyavarietyofmechanisms.
- MahlbergR.etal.Normativedataonthedailyprofileofurinary6-sulfatoxymelatonininhealthysubjectsbetweentheagesof20and84.Psychoneuroendocrinology.2006Jun;31(5):634-41.
- DelaPuertaC.etal.Melatoninisaphytochemicalinoliveoil.FoodChem.2007;104:609-12.
- ChoMH.etal.Chitosantreatmentsaffectgrowthandselectedqualityofsunflowersprouts.JFoodSci.2008Jan;73(1):S70-7.
- MaldonadoMD.etal.Melatoninpresentinbeercontributestoincreasethelevelsofmelatoninandantioxidantcapacityofthehumanserum.ClinNutr.2009Apr;28(2):188-91.
*ThepublicationscanberequestedatIBLinternationalviadan@ibl-international.com
Enzymeimmunoassayforthein-vitrodiagnosticquantitativedeterminationofMelatoninSulfate(synonyms:6-hydroxymelatoninsulfate,6-sulfatoxymelatonin)inhumanurine.Thepinealgland(corpuspineale)hasbeencalledaneuroendocrinetransducerbecauseofitsimportantroleinphotoperiodism.ThemajorhormoneofthepinealglandisN-acetyl-5-methoxy-tryptamineormelatoninwhichissynthezisedfromtheaminoacidtryptophane.Melatoninhasitshighestlevelsinplasmaduringnighttime.Itscharacteristicnocturnalsurgeappearstoencodetemporalinformationsuchaslengthofnight.Regulationofthemelatoninsecretionisunderneuralcontrol.Sympatheticinnervationseemstoplayamajorroleviaitsreleaseofnoradrenaline.Alteredpatternsand/orlevelsofmelatoninsecretionhavebeenreportedtocoincidewithsleepdisorders,jetlag,depression,stress,schizophrenia,hypothalamicamenorrhea,pregnancy,anorexianervosa,someformsofcancer,immunologicaldisordersaswellascontrolofsexualmaturationduringpuberty.Mostofthecirculatingmelatoninismetabolizedintheliverto6-hydroxymelatoninandsubsequentlyto6-sulfatoxymelatoninwhichisexcretedintotheurine.Theconcentrationof6-hydroxymelatoninsulfateinurinecorrelateswellwiththetotallevelofmelatonininthebloodduringthecollectionperiod.
